Mastering Liquid Handling: Recommended Pipette Settings for @Pette Pro & Mini Series
Introduction
Precision in liquid handling is critical across labs, industries, and research. The @Pette Pro and @Pette Mini series smart pipettes offer tailored workflows for different liquid types. This guide breaks down optimal settings to ensure accuracy, efficiency, and consistency—whether you’re working with aqueous solutions, viscous liquids, or hazardous samples.
@Pette Pro Series: Precision for Diverse Liquids
The @Pette Pro adapts to 6 key liquid categories. Below is your quick-reference for speed, mode, tips, and use cases:
| Liquid Type | Aspiration/Dispense Speed | Recommended Mode | Tip Type | Examples & Notes |
|---|---|---|---|---|
| Normal Aqueous | 7–9 | Standard pipetting, dispensing, spotting | Standard | Water, cell media, DNA Marker, 0.1mol/L NaCl. Flexible manual mode for micro-dosing. |
| High Viscosity | 1–3 | Reverse pipetting | Standard/Wide orifice | Glycerol, syrups, Triton X-100. Wide orifices boost speed—mind minor precision tradeoffs. |
| Foam-Prone Liquids | 5–7 | Forward pipetting | Standard | Detergent-based solutions, protein-rich fluids. Minimizes bubble formation. |
| Volatile Compounds | 7–9 | Reverse pipetting | Filtered tips | Ethanol, acetone, ammonia. Filtered tips prevent vapor/liquid backflow. |
| Density-Variant | 7–9 | Reverse pipetting | Standard/Filtered | Benzene, alkanes, CCl₄. Prioritize filtered tips for volatile, low-density liquids. |
| Hazardous Samples | 7–9 | Standard dispensing | Filtered tips | Blood, serum, urine, cultures. Filtered tips contain biohazards, reduce contamination. |
@Pette Mini Series: Streamlined for Routine & Specialty Liquids
The @Pette Mini balances simplicity and adaptability. Use this table to optimize 6 common liquid types:
| Liquid Type | Aspiration/Dispense Speed | Recommended Mode | Tip Type | Examples & Notes |
|---|---|---|---|---|
| Normal Aqueous | 7–9 | Standard pipetting/dispensing | Standard | Water, cell media, 0.1mol/L HCl. Reliable for daily lab workflows. |
| High Viscosity | 1–4 | Reverse pipetting | Standard/Wide orifice | Glycerol, syrups, Triton X-100. Wide orifices improve flow—adjust speed cautiously. |
| Foam-Prone Liquids | 4–9 | Reverse pipetting | Standard | Detergent solutions, protein-rich fluids. Curbs bubble formation. |
| Volatile Compounds | 7–9 | Reverse pipetting | Filtered tips | Ethanol, acetone, ammonia. Filtered tips block vapor ingress. |
| Density-Variant | 7–9 | Reverse pipetting | Standard/Filtered | Benzene, alkanes, CCl₄. Filtered tips safeguard against volatile, low-density fluids. |
| Hazardous Samples | 4–9 | Standard dispensing | Filtered tips | Blood, serum, urine, cultures. Contains biohazards, lowers contamination risk. |
Pro Tips for All Scenarios
1. Tip Selection Matters: Match tip design (standard, wide orifice, filtered) to liquid properties (viscosity, volatility, hazard level).
2. Speed ≠ Compromise: Slow speeds for viscous/high-risk liquids ensure control; faster speeds for aqueous/non-critical tasks save time.
3. Mode Mastery: Reverse pipetting minimizes drips for viscous/foamy liquids; forward mode works best for thin, stable fluids.
Conclusion
The @Pette Pro and Mini series empower labs to handle diverse liquids with confidence. By aligning speed, mode, and tip type to your liquid’s unique traits, you’ll reduce errors, save time, and protect sample integrity. Bookmark this guide for quick reference—your go-to for consistent, precise liquid handling.
