PCR amplification demands precision, consistency, and efficiency—especially in high-throughput labs where even minor errors can derail results. The integration of @Pette Series smart pipettes with automated benchtop workstations creates a seamless workflow that addresses these needs, transforming manual pipetting bottlenecks into a streamlined, reliable process.
1. Synergistic Workflow Design
The @Pette Pro® (single/multi-channel) and @Pette Mini® smart pipettes sync with automated benchtop stations to handle key PCR steps:
- Sample & Reagent Preparation: Multi-channel @Pette Pro® dispenses master mix, primers, and templates into 96/384-well plates with ±0.5% CV precision, while the workstation automates plate handling to avoid cross-contamination.
- Thermal Cycling Integration: Post-pipetting, the workstation transfers plates to thermal cyclers, with @Pette’s digital logs syncing with cycler data for full traceability.
- Post-Amplification Processing: The @Pette Mini®’s microvolume accuracy (down to 0.1µL) ensures precise transfer of amplicons for downstream analysis (e.g., qPCR quantification).
2. Core Advantages for PCR Labs
- Error Reduction: Smart pipettes auto-detect tip misalignment or volume discrepancies, while the workstation eliminates manual plate moves—cutting human error by 70%.
- Throughput Boost: A single setup processes 96 samples in 30 minutes (vs. 2+ hours manually), critical for clinical diagnostics or large-scale screening.
- Consistency: @Pette’s calibrated channels and the workstation’s programmable protocols ensure uniform reaction volumes, reducing CT value variability in qPCR.
- Ergonomics: Automating repetitive pipetting reduces hand strain, a game-changer for labs with high daily sample loads.
3. Real-World Application
A diagnostic lab processing 500+ COVID-19 samples daily reported:
- 40% faster turnaround time after adopting @Pette Pro® 8-channel + automated workstation.
- 99.2% concordance in results (up from 95% with manual pipetting) due to reduced reagent waste and precise volume control.
- Simplified training for new staff, as the system’s pre-set PCR protocols minimize manual input.